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Product Info Summary
| SKU: | A00217S428 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Bovine, Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-Phospho-LKB1 (S428) STK11 Antibody
View all LKB1/STK11 Antibodies
SKU/Catalog Number
A00217S428
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Phospho-LKB1 (S428) STK11 Antibody catalog # A00217S428. Tested in ELISA, IF, IHC-P, WB applications. This antibody reacts with Bovine, Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-LKB1 (S428) STK11 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00217S428)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human LKB1 around the phosphorylation site of S428.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00217S428 is reactive to STK11 in Bovine, Human, Mouse
Applications
A00217S428 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
48.636kDa
Background of LKB1/STK11
PTH elevates calcium level by dissolving the salts in bone and preventing their renal excretion. Stimulates [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblastic cells.
Pranay C. Patel, Arch Otolaryngol Head Neck Surg, May 1998; 124: 559.
Atul A. Gawande, Arch Surg, Apr 2006; 141: 381 - 384.
Timothy M. Murray, Endocr. Rev., Feb 2005; 26: 78 - 113.
W. M. Philbrick, Physiol Rev, Jan 1996; 76: 127.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:40000
Validation Images & Assay Conditions
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Western blot analysis of lysates from HeLa cells treated with PMA 125ng/ml 30', using LKB1 (Phospho-Ser428) Antibody. The lane on the right is blocked with the phospho peptide.
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Immunofluorescence analysis of NIH/3T3 cells, using LKB1 (Phospho-Ser428) Antibody. The picture on the right is blocked with the phospho peptide.
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Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using LKB1 (Phospho-Ser428) Antibody. The picture on the right is blocked with the phospho peptide.
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Immunofluorescence analysis of Hela cell. 1, LKB1 (phospho Ser428) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). NSE monoclonal antibody (13E2) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).
Protein Target Info & Infographic
Gene/Protein Information For STK11 (Source: Uniprot.org, NCBI)
Gene Name
STK11
Full Name
Serine/threonine-protein kinase STK11
Weight
48.636kDa
Superfamily
protein kinase superfamily
Alternative Names
EC 2.7.11.1; LKB1 serine/threonine kinase 11 (Peutz-Jeghers syndrome); LKB1; PJS polarization-related protein LKB1; PJS; Renal carcinoma antigen NY-REN-19; serine/threonine kinase 11; serine/threonine-protein kinase 11; Serine/threonine-protein kinase LKB1; STK11 STK11 LKB1, PJS, hLKB1 serine/threonine kinase 11 serine/threonine-protein kinase STK11|liver kinase B1|polarization-related protein LKB1|renal carcinoma antigen NY-REN-19|serine/threonine-protein kinase 11|serine/threonine-protein kinase LKB1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on STK11, check out the STK11 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for STK11: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-LKB1 (S428) STK11 Antibody (A00217S428)
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No publications found for A00217S428
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